Ulocladium spores, which are excellent indicators of water-damaged conditions, should be differentiated from those of Pithomyces, which is typically of outdoor origin. Particularly note the spores of Chaetomium, Stachybo-trys, Memnoniella, and Ulocladium. Scan for spores over the entire deposit trace. Do not focus the spore counting on a certain section or portion of the adhesive band. If the trace is heavy and thick, rank the load of background particulates on the datasheet. If clustering and clumping of certain spore/conidial types are observed, note it on the datasheet. Determine whether the spore deposit is random or evenly distributed. Use a 10x or 20x objective lens to scan the entire trace of the deposit. We comply with the Federal Trade Commission 1998 Children’s Online Privacy Protection Act (COPPA).1. The 4-H Name and Emblem have special protections from Congress, protected by code 18 USC 707. Reference to commercial products or trade names does not imply endorsement by MSU Extension or bias against those not mentioned. This information is for educational purposes only. Quentin Tyler, Director, MSU Extension, East Lansing, MI 48824. Issued in furtherance of MSU Extension work, acts of May 8 and June 30, 1914, in cooperation with the U.S. Michigan State University Extension programs and materials are open to all without regard to race, color, national origin, gender, gender identity, religion, age, height, weight, disability, political beliefs, sexual orientation, marital status, family status or veteran status. MSU is an affirmative-action, equal-opportunity employer, committed to achieving excellence through a diverse workforce and inclusive culture that encourages all people to reach their full potential. Also if a new infection is not located near the spore trap, the spores will not be picked up by the trap until the disease becomes more widespread. A new infection may not register high spore counts, initially. It is impossible to know where the first outbreak of downy mildew within any particular county may occur. Counting one reel of slides takes 1-2 days.ĭowny mildew spores will be concentrated in areas where the disease is established. Spores (Figure 2F) are counted and logged into a data sheet under the specific hour of the specific day that they were in the air around that particular spore trap. Once the slides are prepared, they are placed under the microscope and the entire tape is examined for downy mildew spores (Figure 2E). A cover slip is applied, sealed to the slide (Figure 2D), and allowed to dry. Tapes from one reel are cut into 7 pieces that represent 24 hours and each hour is marked (Figure 2B).Įach 24-hour section of tape is put onto a microscope slide (Figure 2C) and stained. The tape on the reel is removed and placed onto a cutting block At that time a new reel is placed in the trap, and the reel with the spores is brought back to the lab where it is processed. Processing of one spore trap reel takes approximately 4 hours. The reel inside the spore trap must be changed every 7 days. Each trap operates by continuously pulling in air and all associated particles (spores, pollen, dust, etc.) and impacting them onto sticky tape on a reel inside the trap. Spore traps (Figure 1) are placed in the several sites in Michigan counties for the field season.
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